Change country
Home

Goat Anti-MURF3 / TRIM54 Antibody (EB05883)

Related Products: EBP05883 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05883 Goat Anti-MURF3 / TRIM54 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05883 1
EB05883 staining (1ug/ml) of Human Skeletal Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05883 2
EB05883 (3.75µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM54, MURF-3, tripartite motif-containing 54, RNF30, MURF, ring finger protein 30, muscle-specific RING-finger protein 3, muscle-specific RING-finger protein homolog, OTTHUMP00000122598
Official Symbol: TRIM54
Accession Number(s): NP_115935.3; NP_912730.2
Human GeneID(s): 57159

Immunogen

Peptide with sequence C-GSAGPEEERPDGP, from the C Terminus of the protein sequence according to NP_115935.3; NP_912730.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Experiments gave bands at approx 45kDa and 38kDa in Human Skeletal Muscle lysate after 1µg/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Product Reviews

Please login to review this product.