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Goat Anti-MURF3 / TRIM54 Antibody (EB05883)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05883 | Goat Anti-MURF3 / TRIM54 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human, Dog | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: OTTHUMP00000122598, muscle-specific RING-finger protein homolog, muscle-specific RING-finger protein 3, ring finger protein 30, MURF, RNF30, tripartite motif-containing 54, MURF-3, TRIM54Official Symbol: TRIM54
Accession Number(s): NP_115935.3; NP_912730.2
Human GeneID(s): 57159
Immunogen
Peptide with sequence C-GSAGPEEERPDGP, from the C Terminus of the protein sequence according to NP_115935.3; NP_912730.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:8000.Western blot: Experiments gave bands at approx 45kDa and 38kDa in human skeletal muscle lysate after 1µg/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Dog
Specific References
The goat polyclonal antibody used in this paper was manufactured by us:
Gregorio CC, Perry CN, McElhinny AS.
Functional properties of the titin/connectin-associated proteins, the muscle-specific RING finger proteins (MURFs), in striated muscle.
J Muscle Res Cell Motil. 2005;26(6-8):389-400.
PMID: 16477476
The goat polyclonal antibody used in this paper was manufactured by us:
Khal J, Wyke SM, Russell ST, Hine AV, Tisdale MJ.
Expression of the ubiquitin-proteasome pathway and muscle loss in experimental cancer cachexia.
Br J Cancer. 2005 Oct 3;93(7):774-80.
PMID: 16160695