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Goat Anti-NANOG Antibody (EB06860)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06860 | Goat Anti-NANOG Antibody | Pep-ELISA, WB, IF |  |
100µg specific antibody in 200µl | Human, Dog | $315.00 |  |
|
Ordering Information forOrder direct from Everest
$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: homeobox transcription factor Nanog-delta 48, homeobox transcription factor Nanog, HGNC:20857, Nanog homeobox, NANOGOfficial Symbol: NANOG
Accession Number(s): NP_079141.2
Human GeneID(s): 79923
Non-Human GeneID(s): 71950 (mouse), 414065 (rat)
Immunogen
Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:64000.Western blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.03-0.1µg/ml.
Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). See also publication Raya A. et al., Nature. 2009 Jul 2;460(7251):53-9, PMID: 19483674.
This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Species Reactivity
Tested: Human, PigExpected from sequence similarity: Human, Dog
Specific References
This antibody has been successfully used in the following papers:
Raya A et.al.
Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.
Nature. 2009 Jul 2;460(7251):53-9.
PMID: 19483674
Montserrat N, Garreta E, González F, Gutiérrez J, Eguizábal C, Ramos V, Borrós S, Izpisua Belmonte JC.
Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system.
J Biol Chem. 2011 Apr 8;286(14):12417-28.
PMID: 21285354
Montserrat N, Bahima EG, Batlle L, Häfner S, Rodrigues AM, González F, Belmonte JC.
Generation of pig iPS cells: a model for cell therapy.
J Cardiovasc Transl Res. 2011 Apr;4(2):121-30.
PMID: 21088946
Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, Raya A, Belmonte JC.
Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions.
Stem Cells. 2010 Jan;28(1):36-44.
PMID: 19890879
Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC.
Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.
Nat Biotechnol. 2008 Nov;26(11):1276-84.
PMID: 18931654