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Code Name Applications Availability Size Tested species Price Grade  
EB06860 Goat Anti-NANOG Antibody Pep-ELISA, WB, IF, IHC, EIA Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB06860 1
EB06860 (5ug/ml) staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.
EB06860 2
EB06860 (0.03µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NANOG, Nanog homeobox, HGNC:20857, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Official Symbol: NANOG
Accession Number(s): NP_079141.2
Human GeneID(s): 79923
Non-Human GeneID(s): 71950 (mouse), 414065 (rat)

Immunogen

Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: This antibody was successfully used in Vassena et al, Hum Mol Genet. 2012 Aug 1;21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22;7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012;21(5):815-25. PMID: 21944493.

Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB07693. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig

Specific References

This antibody has been successfully used in IHC on Human:
Sancho-Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, Ma L1, Morey R, Krause MN, Zembrzycki A, Ansorge O5 Vazquez-Ferrer E ,Dubova I1. Reddy P, Lam D, Hishida Y, Wu MZ, Esteban CR, O'Leary D, Wahl GM, Verma IM, Laurent LC, Izpisua Belmonte JC
Establishment of human iPSC-based models for the study and targeting of glioma initiating cells
Nat Commun. 2016 Feb 22;7:10743
PMID: 26899176

This antibody has been successfully used on Human:
Tolar J, McGrath JA, Xia L, Riddle MJ, Lees CJ, Eide C, Keene DR, Liu L, Osborn MJ, Lund TC, Blazar BR, Wagner JE.
Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
J Invest Dermatol. 2014 May;134(5):1246-54.
PMID: 24317394

This antibody has been successfully used in IF on Human:
Montserrat N, Ramírez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, González F, Izpisua Belmonte JC, Campistol JM.
Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2.
J Biol Chem. 2012 Jul 13;287(29):24131-8.
PMID: 22613719

This antibody has been successfully used in IHC on Human:
Vassena R, Montserrat N, Carrasco Canal B, Aran B, de Oñate L, Veiga A, Izpisua Belmonte JC.
Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells.
Hum Mol Genet. 2012 Aug 1;21(15):3366-73.
PMID: 22547223

This antibody has been successfully used in IHC on Pig:
Montserrat N, de Oñate L, Garreta E, González F, Adamo A, Eguizábal C, Häfner S, Vassena R, Izpisua Belmonte JC.
Generation of feeder-free pig induced pluripotent stem cells without Pou5f1.
Cell Transplant. 2012;21(5):815-25.
PMID: 21944493

This antibody has been successfully used on Human:
Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Izpisua Belmonte JC.
Complete meiosis from human induced pluripotent stem cells.
Stem Cells. 2011 Aug;29(8):1186-95.
PMID: 21681858

This antibody has been successfully used in IF on Human:
Raya A et.al.
Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells.
Nature. 2009 Jul 2;460(7251):53-9.
PMID: 19483674

This antibody has been successfully used in ICC/IF on Human:
Montserrat N, Garreta E, González F, Gutiérrez J, Eguizábal C, Ramos V, Borrós S, Izpisua Belmonte JC.
Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system.
J Biol Chem. 2011 Apr 8;286(14):12417-28.
PMID: 21285354

This antibody has been successfully used in IF on Pig:
Montserrat N, Bahima EG, Batlle L, Häfner S, Rodrigues AM, González F, Belmonte JC.
Generation of pig iPS cells: a model for cell therapy.
J Cardiovasc Transl Res. 2011 Apr;4(2):121-30.
PMID: 21088946

This antibody has been successfully used in ICC/IF on Human:
Rodríguez-Pizà I, Richaud-Patin Y, Vassena R, González F, Barrero MJ, Veiga A, Raya A, Belmonte JC.
Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions.
Stem Cells. 2010 Jan;28(1):36-44.
PMID: 19890879

This antibody has been successfully used in ICC/IF on Human:
Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Izpisúa Belmonte JC.
Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes.
Nat Biotechnol. 2008 Nov;26(11):1276-84.
PMID: 18931654

This antibody has been successfully used in ICC on Human:
Cristina Mora, Marialaura Serzanti, Alessio Giacomelli, Valentina Turco, Eleonora Marchina, Valeria Bertini, Giovanna Piovani, Giulia Savio, Lena Refsgaard, Morten Salling Olesen, Venusia Cortellini, Patrizia Dell'Era
Generation of induced pluripotent stem cells (iPSC) from an atrial fibrillation patient carrying a KCNA5 p.D322H mutation
Stem Cell Research 24 (2017) 29-32
PMID: 1

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