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Code Name Applications Availability Size Tested species Price Grade  
EB05054 Goat Anti-NCF1 / p47phox Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Pig $315.00 Elite

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05054 1
EB05054 (0.2µg/ml) staining of U937 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05054 2
EB05054 (1µg/ml) staining of Mouse Thymus (A) and Pig Spleen (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05054 3
EB05054 (5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2
Official Symbol: NCF1
Accession Number(s): NP_000256.3
Human GeneID(s): 653361
Non-Human GeneID(s): 17969 (mouse), 114553 (rat)

Immunogen

Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:20000.
Western blot: Approx 48kDa band observed in lysates of cell line U937 and of Mouse Thymus, and 50kDa observed in Pig Spleen lysates (calculated MW of 44.7kDa according to NP_000256.3). Recommended concentration: 0.5-1.5µg/ml.
IHC: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Pig
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow. This batch is not expected to cross-react with Rat in Western blot.

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