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Goat Anti-NDRG2 Antibody (EB10793)

Code Name Applications Availability Size Tested species Price Grade  
EB10793 Goat Anti-NDRG2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB10793 1
EB10793 (0.1µg/ml) staining of Human Temporal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10793 2
EB10793 (0.3µg/ml) staining of Mouse (A) and Rat (B) Brain lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10793 3
EB10793 (3.75µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NDRG2, NDRG family member 2, DKFZp781G1938, FLJ25522, KIAA1248, SYLD, N-myc downstream regulator 2, NDR1-related protein NDR2, OTTHUMP00000164405, OTTHUMP00000164407, OTTHUMP00000164408, cytoplasmic protein Ndr1, protein NDRG2, syld709
Official Symbol: NDRG2
Accession Number(s): NP_963293.1
Human GeneID(s): 57447
Non-Human GeneID(s): 29811 (mouse), 171114 (rat)
Important Comments: This antibody is expected to recognize reported isoform a (NP_963293.1) only. Reported variants represent identical protein: NP_963831.1, NP_963293.1, NP_963834.1, NP_963833.1.

Immunogen

Peptide with sequence KEAELAARILLDQGQ-C, from the N Terminus (near) of the protein sequence according to NP_963293.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 45kDa band observed in Human Brain (Temporal Cortex), Mouse and Rat Brain lysates (calculated MW of 40.8kDa according to NP_963293.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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