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Goat Anti-NEFL (aa330-343) Antibody (EB13051)

Related Products: EBP13051 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB13051 Goat Anti-NEFL (aa330-343) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB13051 1
EB13051 (1µg/ml) staining of Neuroblastoma KELLY lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NEFL, neurofilament, light polypeptide, CMT1F, CMT2E, NF-L, NF68, NFL, PPP1R110, light molecular weight neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, neurofilament, light polypeptide 68kDa, protein phosphatase 1, regulatory subunit 110
Official Symbol: NEFL
Accession Number(s): NP_006149.2
Human GeneID(s): 4747
Non-Human GeneID(s): 18039 (mouse), 83613 (rat)

Immunogen

Peptide with sequence C-EKQLQELEDKQNAD, from the internal region of the protein sequence according to NP_006149.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 68-70kDa band observed in lysates of Neuroblastoma cell line KELLY (calculated MW of 61.5kDa according to NP_006149.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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