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Code Name Applications Availability Size Tested species Price Grade  
EB06775 Goat Anti-NGFR Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06775 1
EB06775 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NGFR, nerve growth factor receptor (TNFR superfamily, member 16), HGNC:7809, TNFRSF16, p75(NTR), p75NTR, CD271, low affinity nerve growth factor receptor, low-affinity nerve growth factor receptor, Gp80-LNGFR, nerve growth factor receptor
Official Symbol: NGFR
Accession Number(s): NP_002498.1
Human GeneID(s): 4804

Immunogen

Peptide with sequence C-AKREEVEKLLNG, from the internal region of the protein sequence according to NP_002498.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75kDa band observed in Human Duodenum and Human Heart lysates (calculated MW of 45.2kDa according to NP_4804). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nykjaer et al, Curr Opin Neurobiol. 2005 Feb;15(1):49-57). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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