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Goat Anti-Nogo 66 receptor Antibody (EB06758)

Related Products: EBP06758 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06758 Goat Anti-Nogo 66 receptor Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06758 1
EB06758 (0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Nogo-66 receptor, RTN4R, reticulon 4 receptor, HGNC:1860, NGR, NOGOR, UNQ330/PRO526, nogo receptor, reticulon 4 receptor precursor, OTTHUMP00000197866
Official Symbol: RTN4R
Accession Number(s): NP_075380.1
Human GeneID(s): 65078

Immunogen

Peptide with sequence C-LQALPDDTFRDLGN, from the internal region of the protein sequence according to NP_075380.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Experiments gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate after 0.3µg/ml antibody staining. These bands were successfully blocked by the immunizing peptide and they correspond to earlier findings in the literature with different antibodies (Wang et al, J Neurosci. 2002 Jul 1;22(13):5505-15). This protein has a calculated MW of 50.7kDa according to NP_075380.1. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog

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