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Goat Anti-NOS1 Antibody (EB05259)

Code Name Applications Availability Size Tested species Price Grade  
EB05259 Goat Anti-NOS1 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05259 1
EB05259 (2µg/ml) staining of Human Frontal Cortex (A) and (1ug/ml) Brain Sub Ventricular Zone (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05259 2
EB05259 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and Plasma Membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB05259 3
EB05259 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB05259 4
EB05259 (5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS, IHPS1, nNOS, nitric oxide synthase 1, neuronal
Official Symbol: NOS1
Accession Number(s): NP_000611.1; NP_001191147.1; NP_001191142.1
Human GeneID(s): 4842

Immunogen

Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1; NP_001191147.1; NP_001191142.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 150kDa band observed in Human Brain Frontal Cortex and Brain Sub Ventricular Zone lysates (calculated MW of 161kDa according to NP_000611.1) The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.

Immunofluorescence: Strong expression of the protein seen in the nucleus and plasma membrane of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody (previous batch) has been successfully used in IF on Mouse:
Fried HU, Kaupp UB, Müller F.
Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice.
Cell Tissue Res. 2010 Mar;339(3):463-79.
PMID: 20140458

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