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Goat Anti-NOS1 Antibody (EB05259)

Code Name Applications Availability Size Tested species Price Grade  
EB05259 Goat Anti-NOS1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05259 1
EB05259 (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05259 2
EB05259 (5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NOS1, nitric oxide synthase 1 (neuronal), NOS, neuronal nitric oxide synthase, PnNOS, penile neuronal nitric oxide synthase, penile neuronal NOS, IHPS1, nNOS, nitric oxide synthase 1, neuronal
Official Symbol: NOS1
Accession Number(s): NP_000611.1; NP_001191147.1; NP_001191142.1
Human GeneID(s): 4842

Immunogen

Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1; NP_001191147.1; NP_001191142.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 160-170kDa band observed in Human Skeletal Muscle lysates (calculated MW of 161kDa according to NP_000611.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. Preliminary testing was unsuccessful on Mouse, Rat Brain and Rat Skeletal Muslce for this particular batch.
IHC: Paraffin embedded Human Colon (Myenteric Plexus). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in IHC and IF on Mouse:
Fried HU, Kaupp UB, Müller F.
Hyperpolarization-activated and cyclic nucleotide-gated channels are differentially expressed in juxtaglomerular cells in the olfactory bulb of mice.
Cell Tissue Res. 2010 Mar;339(3):463-79.
PMID: 20140458

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