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Code Name Applications Availability Size Tested species Price Grade  
EB05370 Goat Anti-NQO1 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Rat, Pig $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05370 1
EB05370 staining (0.03µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05370 2
EB05370 (1µg/ml) staining of Rat (A) and Pig (B) Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence
EB05370 3
EB05370 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05370 4
EB05370-C3 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H menadione oxidoreductase 1, dioxin-inducible, NAD(P)H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1
Official Symbol: NQO1
Accession Number(s): NP_000894.1; NP_001020604.1; NP_001020605.1
Human GeneID(s): 1728
Non-Human GeneID(s): 18104 (mouse)
Important Comments: This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).

Immunogen

Peptide with sequence C-SIPTDNQIKARK, from the C Terminus of the protein sequence according to NP_000894.1; NP_001020604.1; NP_001020605.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 30kDa band observed in Human Kidney and Lung lysates and in Rat and Pig Kidney lysates (calculated MW of 30.9kDa according to NP_000894.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Rat, Pig
Expected from sequence similarity: Human, Rat, Dog, Pig

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