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Goat Anti-OGT Antibody (EB07626)

Code Name Applications Availability Size Tested species Price Grade  
EB07626 Goat Anti-OGT Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $315.00 Elite

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ARP American Research Products, Inc
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EB07626 1
EB07626 (0.05µg/ml) staining of Rat Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07626 2
EB07626 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: OGT, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase), FLJ23071, HRNT1, MGC22921, O-GLCNAC, O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
Official Symbol: OGT
Accession Number(s): NP_858058.1; NP_858059.1
Human GeneID(s): 8473
Non-Human GeneID(s): 108155 (mouse), 26295 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_858058.1 and NP_858059.1


Peptide with sequence C-YEHPKDLKLSDGR, from the internal region of the protein sequence according to NP_858058.1; NP_858059.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 110kDa band observed in Pancreas lysates from Rat (calculated MW of 117kDa according to NP_858058.1. Recommended concentration: 0.05-0.2µg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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