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Goat Anti-ORAI1 / CRACM1 Antibody (EB09022)

Code Name Applications Availability Size Tested species Price Grade  
EB09022 Goat Anti-ORAI1 / CRACM1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB09022 1
EB09022 (0.1µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). . Detected by chemiluminescence.
EB09022 2
EB09022 (0.03µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ORAI1, CRACM1, ORAI calcium release-activated calcium modulator 1, FLJ14466, ORAT1, TMEM142A, calcium release-activated calcium channel protein 1, calcium release-activated calcium modulator 1, transmembrane protein 142A
Official Symbol: ORAI1
Accession Number(s): NP_116179.2
Human GeneID(s): 84876
Non-Human GeneID(s): 109305 (mouse)

Immunogen

Peptide with sequence C-KKQPGQPRPTSKP, from the internal region of the protein sequence according to NP_116179.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 50-55kDa band observed in Human Placenta and Skin lysates (calculated MW of 32.7kDa according to NP_116179.2). This molecular weight is routinely observed by other sources e.g. Li et al,; PMID: 17702753. Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig

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