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Goat Anti-ORP5 (OBPH1) Antibody (EB06154)

Related Products: EBP06154 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06154 Goat Anti-ORP5 (OBPH1) Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human, Rat $464.00 Elite_Plus

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EB06154 1
EB06154 (0.1µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06154 2
EB06154 (0.3µg/ml) staining of Rat Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06154 3
EB06154 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06154 4
EB06154 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06154 5
EB06154 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06154 6
EB06154 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06154 7
EB06154 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5
Official Symbol: OSBPL5
Accession Number(s): NP_065947.1; NP_663613.1; NP_001137535.1
Human GeneID(s): 114879
Non-Human GeneID(s): 79196 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.

Immunogen

Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1; NP_663613.1; NP_001137535.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 100kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4kDa according to Human NP_065947.1 and 101.7kDa according to Rat NP_001015024.2). Recommended concentration: 0.03=0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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