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Goat Anti-OSMR Antibody (EB09898)

Related Products: EBP09898 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09898 Goat Anti-OSMR Antibody Pep-ELISA, WB, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09898 1
EB09898 (1µg/ml) staining of U937 (A) and (0.3ug/ml) A431 (B) and HepG2 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09898 2
EB09898 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: MGC150626, MGC150627, MGC75127, oncostatin M receptor, OSMRB, OSMR
Official Symbol: OSMR
Accession Number(s): NP_003990.1; NP_001161827.1; NP_001310435.1; NP_001310436.1
Human GeneID(s): 9180
Non-Human GeneID(s): 18414 (mouse)
Important Comments: The immunizing peptide represents part of the extracellular domain.

Immunogen

Peptide with sequence C-QIHGEQLDPHVT, from the internal region of the protein sequence according to NP_003990.1; NP_001161827.1; NP_001310435.1; NP_001310436.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 125kDa band observed in lysates of cell line U937 and approx 70+125kDa in lysates of cell line A431 and HepG2 (calculated MW of 111kDa according to NP_003990.1 (isoform 1) and NP_001310435.1 (isoform 3), and 66.0kDa according to NP_001310436.1 (isoform 4)). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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