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Goat Anti-OXT Antibody (EB09023)

Code Name Applications Availability Size Tested species Price Grade  
EB09023 Goat Anti-OXT Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB09023 1
EB09023 (0.05µg/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
Click here to download PDF datasheet.

Target Protein

Principal Names: OXT, oxytocin, prepro- (neurophysin I), MGC126890, MGC126892, OT, OT-NPI, oxytocin-neurophysin I, oxytocin-neurophysin I, preproprotein
Official Symbol: OXT
Accession Number(s): NP_000906.1
Human GeneID(s): 5020

Immunogen

Peptide with sequence HADPACDAEATFSQR, from the C Terminus of the protein sequence according to NP_000906.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments in Human Brain (Cerebellum, Amygdala) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: Frozen section of Human Hypothalamus shows staining of magnocellular oxytocin neurons in the supraoptic nucleus. Recommended concentration, 0.05-0.15µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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