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Code Name Applications Availability Size Tested species Price Grade  
EB05880 Goat Anti-PARK7 / DJ-1 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $450.00 Elite_Plus

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05880 1
EB05880 (0.001µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05880 2
EB05880 (0.001µg/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05880 3
Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml EB05880 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.
EB05880 4
EB05880 (7µg/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05880 5
EB05880 Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PARK7, DJ1, DJ-1, Parkinson disease (autosomal recessive, early onset) 7, oncogene DJ1, CTA-215D11.1, FLJ27376, FLJ34360, FLJ92274, Parkinson disease protein 7, protein DJ-1, epididymis secretory sperm binding protein Li 67p, HEL-S-67p
Official Symbol: PARK7
Accession Number(s): NP_009193.2
Human GeneID(s): 11315
Non-Human GeneID(s): 57320 (mouse), 117287 (rat)
Important Comments: Reported variants (NP_001116849.1; NP_009193.2) represent the same protein.

Immunogen

Peptide with sequence C-AAQVKAPLVLKD, from the C Terminus of the protein sequence according to NP_009193.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 24kDa band observed in lysates of cell lines HeLa and Jurkat and of Human Brain (Cerebellum, Hippocampus, Frontal Cortex) and rodent Brain lysates (calculated MW of 19.9kDa according to NP_001116849.1 and of NP_009193.2). Recommended concentration: 0.05-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis. Recommended concentration: 7µg/ml.

Immunofluorescence: Strong expression of the protein seen around the nucleus. Dividing cells appear to show enhanced expression. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

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