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Goat Anti-PDGFB precursor Antibody (EB09553)

Related Products: EBP09553 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09553 Goat Anti-PDGFB precursor Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09553 1
EB09553 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB09553 2
EB09553 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PDGFB, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), FLJ12858, PDGF2, SIS, SSV, c-sis, PDGF, B chain, Platelet-derived growth factor, beta polypeptide (oncogene SIS), becaplermin, oncogene SIS, platelet-derived growth factor 2, platelet-derived growth factor beta, platelet-derived growth factor, B chain, v-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog)
Official Symbol: PDGFB
Accession Number(s): NP_002599.1; NP_148937.1
Human GeneID(s): 5155
Non-Human GeneID(s): 18591 (mouse), 24628 (rat)
Important Comments: This antibody is expected to recognize isoform 1 and 2 (NP_002599.1; NP_148937.1).

Immunogen

Peptide with sequence C-KHRKFKHTHDKT, from the C Terminus of the protein sequence according to NP_002599.1; NP_148937.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.

Immunofluorescence: Strong expression of the protein seen in the vesicles of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep

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