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Goat Anti-Phenylalanine Hydroxylase Antibody (EB10324)

Code Name Applications Availability Size Tested species Price Grade  
EB10324 Goat Anti-Phenylalanine Hydroxylase Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
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  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB10324 1
EB10324 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10324 2
EB10324 (0.3µg/ml) staining of Rat Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PH, phenylalanine hydroxylase, PKU, PKU1, PAH
Official Symbol: PAH
Accession Number(s): NP_000268.1
Human GeneID(s): 5053
Non-Human GeneID(s): 18478 (mouse), 24616 (rat)

Immunogen

Peptide with sequence C-ESRPSRLKKDE, from the internal region of the protein sequence according to NP_000268.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 48kDa band observed in Human Liver lysates and approx. 50-52kDa in Rat Kidney lysates and in preliminary testing of Mouse Liver lysate (calculated MW of 51.9kDa according to Hyman NP_000268.1 and Rat NP_036751.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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