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Goat Anti-phospho-CD244 / 2B4 Antibody (EB03004)

Code Name Applications Availability Size Tested species Price Grade  
EB03004 Goat Anti-phospho-CD244 / 2B4 Antibody Pep-ELISA, IP Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB03004 1
EB03004 (2µg/ml) staining of IP from a lysate of Human Natural Killer cells with (+) or without (-) pervanadate treatment of the cells. Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr Nicholas G Clarkson, Sir William Dunn School of Pathology, Oxford, UK
Click here to download PDF datasheet.

Target Protein

Principal Names: CD244, CD244 molecule, natural killer cell receptor 2B4, 2B4, NAIL, NKR2B4, Nmrk, SLAMF4, CD244 natural killer cell receptor 2B4, NK cell activation inducing ligand NAIL, natural killer cell receptor 2B4
Official Symbol: CD244
Accession Number(s): NP_057466.1
Human GeneID(s): 51744

Immunogen

Peptide with sequence C-EFLTIpYEDVKD, from the internal region of the protein sequence according to NP_057466.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 75kDa band observed in Human Natural Killer (NK) cell lysates after immunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose (calculated MW of 41.1kDa according to NP_057466.1). We do note that CD244 is a glycoprotein which makes a higher size band to be expected. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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