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Goat Anti-PIST / FIG / GOPC Antibody (EB06239)

Related Products: EBP06239 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06239 Goat Anti-PIST / FIG / GOPC Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Dog, Cow $315.00 Elite

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EB06239 1
EB06239 (0.05µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: dJ94G16.2 PIST, dJ94G16.2, GOPC1, FIG, CAL, golgi associated PDZ and coiled-coil motif containing, Golgi associated PDZ and coiled-coil motif containing protein, CFTR-associated ligand, fused in glioblastoma, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, CAL, GOPC, PIST
Official Symbol: GOPC
Accession Number(s): NP_065132.1; NP_001017408.1
Human GeneID(s): 57120
Important Comments: This antibody is expected to recognize both reported isoforms (NP_065132.1; NP_001017408.1).

Immunogen

Peptide with sequence C-LDDLHTLYHKKSY, from the C Terminus of the protein sequence according to NP_065132.1; NP_001017408.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 55kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 50.5kDa according to NP_065132.1). Recommended concentration: 0.05-0.2µg/ml.

Immunofluorescence: This product has been successfully used on HeLa cells.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

Specific Reference

The goat antibody described in this paper has been manufactured by us
Hicks SW, Machamer CE.
Isoform-specific interaction of golgin-160 with the Golgi-associated protein PIST.
J Biol Chem. 2005 Aug 12;280(32):28944-51.
PMID: 15951434

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