Change country
Home

Goat Anti-PKD1 / Polycystin 1 Antibody (EB08670)

Code Name Applications Availability Size Tested species Price Grade  
EB08670 Goat Anti-PKD1 / Polycystin 1 Antibody Pep-ELISA, IHC, IP, IF Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB08670 1
EB08670 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
EB08670 2
EB08670 (3.75µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PKD1, polycystic kidney disease 1 (autosomal dominant), PBP, polycystic kidney disease-associated protein, polycystin 1
Official Symbol: PKD1
Accession Number(s): NP_001009944.1; NP_000287.2
Human GeneID(s): 5310
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001009944.1 and NP_000287.2).

Immunogen

Peptide with sequence C-RTPLRAKNKVHP, from the C Terminus of the protein sequence according to NP_001009944.1; NP_000287.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
IHC: In paraffin embedded Human Kidney shows textured cytoplasmic staining towards the basal membrane in PCT. Recommended concentration: 3-5µg/ml. Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml.

Immunoprecipitation: This antibody was successfully used on Human (PMID:25574838, and PMID: 29706351). Immunofluorescence: This antibody was successfully used in IF on Human (Durand et al, Sci Rep. 2016 Oct 24;6:35963, PMID: 27775029).

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific References

This antibody has been successfully used in IP on Human:
Cornec-Le Gall E, Olson RJ, Besse W, Heyer CM, Gainullin VG, Smith JM, Audrézet MP, Hopp K, Porath B, Shi B, Baheti S, Senum SR, Arroyo J, Madsen CD, Férec C, Joly D, Jouret F, Fikri-Benbrahim O, Charasse C, Coulibaly JM, Yu AS, Khalili K, Pei Y, Somlo S, Le Meur Y, Torres VE; Genkyst Study Group; HALT Progression of Polycystic Kidney Disease Group; Consortium for Radiologic Imaging Studies of Polycystic KidneyDisease, Harris PC.
Monoallelic Mutations to DNAJB11 Cause Atypical Autosomal-Dominant Polycystic Kidney Disease.
Am J Hum Genet. 2018 May 3;102(5):832-844
PMID: 29706351

This antibody has been successfully used in IF on Human:
Durand N, Bastea LI, Long J, Döppler H, Ling K, Storz P.
Protein Kinase D1 regulates focal adhesion dynamics and cell adhesion through Phosphatidylinositol-4-phosphate 5-kinase type-l γ.
Sci Rep. 2016 Oct 24;6:35963.
PMID: 27775029

This antibody has been successfully used in IP on Human:
Gainullin VG, Hopp K, Ward CJ, Hommerding CJ, Harris PC.
Polycystin-1 maturation requires polycystin-2 in a dose-dependent manner.
J Clin Invest. 2015 Jan 9. pii: 76972.
PMID: 25574838

Product Reviews

Please login to review this product.