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Goat Anti-POU4F2 Antibody (EB10032)

Code Name Applications Availability Size Tested species Price Grade  
EB10032 Goat Anti-POU4F2 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $210.00 Aspiring

Ordering Information for

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$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

Target Protein

Principal Names: BRN3.2, BRN3B, Brn-3b, Brn3b POU domain transcription factor, POU class 4 homeobox 2, POU domain class 4 transcription factor 2, POU domain protein, POU domain, class 4, transcription factor 2, POU4F2
Official Symbol: POU4F2
Accession Number(s): NP_004566.2
Human GeneID(s): 5458
Important Comments: This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)

Immunogen

Peptide with sequence HGLPSHMGCMSDVD, from the internal region of the protein sequence according to NP_004566.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 70kDa band in Rat Retina lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.2kDa according to NP_599182.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.

Species Reactivity

Tested:
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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