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Goat Anti-PPP2CA / PPP2CB Antibody (EB05230)

Related Products: EBP05230 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05230 Goat Anti-PPP2CA / PPP2CB Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Rabbit, Pig, Cow $315.00 Elite

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EB05230 1
EB05230 (0.05µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform, protein phosphatase type 2A catalytic subunit, protein phosphatase 2, catalytic subunit, beta isoform, PP2A-beta, PP2CB, protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform, PPP2CB, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform, replication protein C, protein phosphatase 2, catalytic subunit, alpha isoform, PP2A-alpha, RP-C, PP2CA, PP2Ac, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, PPP2CA
Official Symbol: PPP2CA / PPP2CB
Accession Number(s): NP_002706.1; NP_004147.1
Human GeneID(s): 5515 , 5516
Non-Human GeneID(s): 19052 (mouse), 24672 (rat)
Important Comments: Two variants NP_001009552.1 and NP_004147.1 represent identical protein.

Immunogen

Peptide with sequence C-EPHVTRRTPDYFL, from the C Terminus of the protein sequence according to NP_002706.1; NP_004147.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in Human Brain (Cerebellum), Mouse Brain and Rat Brain lysates (calculated MW of 35.6kDa according to NP_002706.1and NP_004147.1). Recommended concentration: 0.05-0.2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Rabbit, Pig, Cow

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