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Goat Anti-PPP2R3A Antibody (EB08233)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB08233 | Goat Anti-PPP2R3A Antibody | Pep-ELISA |  |
100µg specific antibody in 200µl | Human, | $210.00 |  |
|
Ordering Information forOrder direct from Everest
$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be processed and invoiced by our US partner Abcore LLC. Order offlineNow shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:
In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: Serine/threonine protein phosphatase 2A, 72/130 kDa regul, PP2A, subunit B, R3 isoform, PP2A, subunit B, B72/B130 isoforms, PP2A, subunit B, B\1\1-PR72/PR130, PR72, PR130, PPP2R3, protein phosphatase 2 (formerly 2A), regulatory subunit B\1\1, alpha, PPP2R3AOfficial Symbol: PPP2R3A
Accession Number(s): NP_002709.2; NP_871626.1
Human GeneID(s): 5523
Important Comments: This antibody is expected to recognise both reported isoforms (NP_002709.2; NP_871626.1).
Immunogen
Peptide with sequence C-QKDVENDGPEPSD, from the internal region of the protein sequence according to NP_002709.2; NP_871626.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: Preliminary experiments gave an approx 80kDa band in Human Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130kDa according to NP_002709.2 and of 61.1kDa according to NP_871626.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species Reactivity
Tested:Expected from sequence similarity: Human,