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Goat Anti-PPP2R5E Antibody (EB05231)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05231 Goat Anti-PPP2R5E Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB05231 1
EB05231 staining (0.5µg/ml) of mouse brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon, epsilon isoform of regulatory subunit B56, protein phosphatase 2A, PP2A, B subunit, R5 epsilon, protein phosphatase 2, regulatory subunit B\1, epsilon isoform, Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon isoform, PP2A, B subunit, PR61 epsilon isoform, PP2A, B subunit, B56 epsilon isoform, PP2A, B subunit, R5 epsilon isoform, PP2A, B subunit, B\1 epsilon isoform, protein phosphatase 2, regulatory subunit B (B56), epsilon isoform, PPP2R5E
Official Symbol: PPP2R5E
Accession Number(s): NP_006237.1
Human GeneID(s): 5529

Immunogen

Peptide with sequence C-LKRGLRRDGIIPT, from the C Terminus of the protein sequence according to NP_006237.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa and 40kDa bands seen in mouse brain extracts. Recommended for use at 0.5-2µg/ml. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36):22081-9).

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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