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Code Name Applications Availability Size Tested species Price Grade  
EB11585 Goat Anti-PRKCB Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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EB11585 1
EB11585 (0.3µg/ml) staining of Human Placenta (A) and Rat Brain (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11585 2
EB11585 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MGC41878, OTTHUMP00000162377, OTTHUMP00000162378, PKCB, PKC-B, PKC-beta, PRKCB, PRKCB1, PRKCB2, protein kinase C beta type, protein kinase C, beta, protein kinase C, beta 1 polypeptide
Official Symbol: PRKCB
Accession Number(s): NP_997700.1
Human GeneID(s): 5579
Important Comments: This antibody is expected to recognize isoform 1 (NP_997700.1) only.

Immunogen

Peptide with sequence C-DKEFTRQPVELT, from the internal region (near C terminus) of the protein sequence according to NP_997700.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 80kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9kDa according to NP_997700.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Rat, Dog

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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