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Goat Anti-PRL3 / PTP4A3 Antibody (EB05302)

Related Products: EBP05302 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05302 Goat Anti-PRL3 / PTP4A3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

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EB05302 1
EB05302 staining (0.03µg/ml) of Human Pancreas lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: potentially prenylated protein tyrosine phosphatase, PRL-R, PRL-3, protein tyrosine phosphatase type IVA, member 3, PRL3, PTP4A3
Official Symbol: PTP4A3
Accession Number(s): NP_009010.2; NP_116000.1
Human GeneID(s): 11156
Non-Human GeneID(s): 19245 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_009010.2 and NP_116000.1).

Immunogen

Peptide with sequence NRPAPVEVSYKHMR-C, from the N Terminus of the protein sequence according to NP_009010.2; NP_116000.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 22kDakDa band observed in Human Pancreas lysates (calculated MW of 19.5Da according to NP_116000.1). Recommended concentration: 0.03-0.1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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FAQs

Q: Is there cross-reactivty expected with PRL1?

A: Probably yes, because the immuzing peptide shows a close match, albeit not 100%, with PRL1

Query 1 NRPAPVEVSYKHMR 14
               NRPAPVEV   YK  MR
Sbjct 5  NRPAPVEVTYKNMR 18