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Code Name Applications Availability Size Tested species Price Grade  
EB07166 Goat Anti-PRODH Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB07166 1
EB07166 (3.75µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07166 2
EB07166 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07166 3
EB07166 (3.75µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRODH, proline dehydrogenase (oxidase) 1, HSPOX2, PIG6, PRODH1, PRODH2, SCZD4, TP53I6, FLJ33744, MGC148078, MGC148079, Proline oxidase, mitochondrial, p53 induced protein, proline dehydrogenase (proline oxidase ), proline oxidase 2, tumor protein p53 inducible protein 6
Official Symbol: PRODH
Accession Number(s): NP_057419.2
Human GeneID(s): 5625
Non-Human GeneID(s): 19125 (mouse)

Immunogen

Peptide with sequence C-ERDGSGTNKRDKQYQ, from the internal region of the protein sequence according to NP_057419.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: This antibody has been successfully used in westen blot on MCF-7 cells: Zareba et al, Oncotarget. 2018 Feb 9;9(17):13748-13757, PMID: 29568391.
IHC: In paraffin embedded Cerebellum shows mitochondrial staining in smaller neurons. Paraffin embedded Human Liver and Colon. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

Specific Reference

This antibody has been successfully used in Western blot on Human:
Ilona Zareba, Katarzyna Celinska-Janowicz, Arkadiusz Surazynski, Wojciech Miltyk and Jerzy Palka
Proline oxidase silencing induces proline-dependent pro survival pathways in MCF-7 cells
Oncotarget. 2018 Feb 9;9(17):13748-13757
PMID: 29568391

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