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Code Name Applications Availability Size Tested species Price Grade  
EB07166 Goat Anti-PRODH Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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EB07166 1
EB07166 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07166 2
EB07166 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRODH, proline dehydrogenase (oxidase) 1, HSPOX2, PIG6, PRODH1, PRODH2, SCZD4, TP53I6, FLJ33744, MGC148078, MGC148079, Proline oxidase, mitochondrial, p53 induced protein, proline dehydrogenase (proline oxidase ), proline oxidase 2, tumor protein p53 inducible protein 6
Official Symbol: PRODH
Accession Number(s): NP_057419.2
Human GeneID(s): 5625
Non-Human GeneID(s): 19125 (mouse)

Immunogen

Peptide with sequence C-ERDGSGTNKRDKQYQ, from the internal region of the protein sequence according to NP_057419.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 6-7µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse

Specific References

This antibody (previous batch) has been successfully used in Western blot on Human:
Ilona Oscilowska, Thi Y L Huynh, Weronika Baszanowska, Izabela Prokop, Arkadiusz Surazynski, Mauro Galli, Piotr Zabielski, Jerzy Palka
Proline oxidase silencing inhibits p53-dependent apoptosis in MCF-7 breast cancer cells
Amino Acids. 2021 Dec;53(12):1943-1956.
PMID: 34085157

This antibody (previous batch) has been successfully used in Western blot on Human:
Natalia Tołoczko-Iwaniuk, Dorota Dziemianczyk-Pakieła, Katarzyna Celinska-Janowicz, Ilona Zareba, Agnieszka Klupczynska, Zenon J. Kokot, Beata Klaudia Nowaszewska, Joanna Reszec, Jan Borys and Wojciech Miltyk
Proline-Dependent Induction of Apoptosis in Oral Squamous Cell Carcinoma (OSCC)—The Effect of Celecoxib
Cancers 2020, 12(1), 136; https://doi.org/10.3390/cancers12010136
PMID: 31935820

This antibody (previous batch) has been successfully used in Western blot on Human:
Ilona Zareba, Katarzyna Celinska-Janowicz, Arkadiusz Surazynski, Wojciech Miltyk and Jerzy Palka
Proline oxidase silencing induces proline-dependent pro survival pathways in MCF-7 cells
Oncotarget. 2018 Feb 9;9(17):13748-13757
PMID: 29568391

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