Change country
Tel: 888-320-GOAT (4628)
Fax: 888-841-9041
Home

Goat Anti-Proopiomelanocortin / POMC Antibody (EB06727)

Related Products: EBP06727 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06727 Goat Anti-Proopiomelanocortin / POMC Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $315.00 Elite

Ordering Information for

Order direct from Everest

$40.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are placed with and invoiced by our US partner Abcore LLC.

 
EB06727 1
EB06727 (0.01µg/ml) staining of NIH 3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06727 2
EB06727 (2.5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: proopiomelanocortin preproprotein, pro-ACTH-endorphin, met-enkephalin, melanotropin gamma, melanotropin beta, melanotropin alpha, lipotropin gamma, lipotropin beta, gamma-MSH, gamma-LPH, corticotropin-like intermediary peptide, beta-melanocyte-stimulating hormone, beta-MSH, beta-LPH, alpha-melanocyte-stimulating hormone, adrenocorticotropin, adrenocorticotropic hormone, OTTHUMP00000200964, OTTHUMP00000119991, NPP, LPH, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin, proopiomelanocortin, POMC
Official Symbol: POMC
Accession Number(s): NP_000930.1; NP_001030333.1
Human GeneID(s): 5443
Important Comments: Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only.

Immunogen

Peptide with sequence C-NAIIKNAYKKGE, from the C Terminus of the protein sequence according to NP_000930.1; NP_001030333.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 26kDa band observed in lysates of Mouse cell line NIH3T3 ((calculated MW of 29.4kDa according to Human NP_000930.1 and of 26.7kDa according to Mouse NP_032921.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: In paraffin embedded Human Adrenal Gland shows strong cytoplasm staining in select cells in the medulla. Recommended concentration, 2-4µg/ml. Data obtained from previous batch.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

Product Reviews

Please login to review this product.