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Goat Anti-PTBP1 / PTB Antibody (EB06088)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06088 Goat Anti-PTBP1 / PTB Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Pig, Cow $315.00 Elite

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EB06088 1
EB06088 (0.1µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06088 2
EB06088 (0.01µg/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06088 3
EB06088 (5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: PTBP1 antibody, PTB antibody, polypyrimidine tract binding protein 1 antibody, PTB2 antibody, PTB3 antibody, PTB4 antibody, pPTB antibody, HNRPI antibody, PTB-1 antibody, HNRNPI antibody, RNA binding protein antibody, heterogeneous nuclear ribonucleoprotein polypeptide I antibody, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody, HNRNP-I antibody, MGC10830 antibody, MGC8461 antibody, RNA-binding protein antibody, heterogeneous nuclear ribonucleoprotein I antibody
Official Symbol: PTBP1
Accession Number(s): NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1
Non-Human GeneID(s): 19205 (mouse), 29497 (rat)
Important Comments: This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).

Immunogen

Peptide with sequence DGIVPDIAVGTKR-C, from the N Terminus of the protein sequence according to NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in Human Liver and Ovary lysates, and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and HEK293 (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Data obtained from a previous batch (from different goat). Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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