Change country
Code Name Applications Availability Size Tested species Price Grade  
EB07025 Goat Anti-PTCH (C Terminus) Antibody Pep-ELISA, IHC. IF Yes 100µg specific antibody in 200µl Human $315.00 Elite

Ordering Information for

Order direct from Everest

$49.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (, fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.



Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613

EB07025 1
EB07025 (3.8µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07025 2
EB07025 (3.75µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB07025 3
EB07025 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
Click here to download PDF datasheet.

Target Protein

Principal Names: PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, PTCH1, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b, PTCH protein +4', PTCH protein -10
Official Symbol: PTCH1
Accession Number(s): NP_000255.1; NP_001077072.1; NP_001077071.1; NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1
Human GeneID(s): 5727
Important Comments: This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.


Peptide with sequence ELQDVECEERPR, from the C Terminus of the protein sequence according to NP_000255.1; NP_001077072.1; NP_001077071.1; NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
IHC: In paraffin embedded Human Colon shows textured nuclear staining of the absorptive cells. Recommended concentration: 3-6µg/ml. Paraffin embedded Human Breast. Recommended concentration: 3.75µg/ml.

Immunofluorescence:Weak expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific References

This antibody has been successfully used in IHC and IF in Human:
Abiria SA, Williams TV, Munden AL, Grover VK, Wallace A, Lundberg CJ, Valadez JG, Cooper MK.
Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling.
J Neurooncol. 2014 May 28.
PMID: 24867209

This antibody has been successfully used in IHC in Human:
Gerardo Valadez J, Grover VK, Carter MD, Calcutt MW, Abiria SA, Lundberg CJ, Williams TV, Cooper MK.
Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation.
Cancer Lett. 2013 Jan 28;328(2):297-306.
PMID: 23063752

Product Reviews

Please login to review this product.