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|EB07025||Goat Anti-PTCH (C Terminus) Antibody||Pep-ELISA, IHC, IF||100µg specific antibody in 200µl||Human, Mouse||$315.00|
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Peptide with sequence ELQDVECEERPR, from the C Terminus of the protein sequence according to NP_000255.1; NP_001077072.1; NP_001077071.1; NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Immunofluorescence:Expression of the protein seen in the cytoplasm of HeLa and in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5µg/ml.
This antibody has been successfully used in IHC and IF in Human:
Abiria SA, Williams TV, Munden AL, Grover VK, Wallace A, Lundberg CJ, Valadez JG, Cooper MK.
Expression of Hedgehog ligand and signal transduction components in mutually distinct isocitrate dehydrogenase mutant glioma cells supports a role for paracrine signaling.
J Neurooncol. 2014 May 28.
This antibody has been successfully used in IHC in Human:
Gerardo Valadez J, Grover VK, Carter MD, Calcutt MW, Abiria SA, Lundberg CJ, Williams TV, Cooper MK.
Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation.
Cancer Lett. 2013 Jan 28;328(2):297-306.