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Goat Anti-PTEN Antibody (EB06544)

Code Name Applications Availability Size Tested species Price Grade  
EB06544 Goat Anti-PTEN Antibody Pep-ELISA, WB, IP Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US partner Abcore LLC.

 

OR

Order from our local distributor

Labscoop

Little Rock, Arkansas
P.O. Box 242164

Email: support@labscoop.com
Website: https://www.labscoop.com/
Phone: 800.316.3081
Fax: 800.316.3081

EB06544 1
EB06544 (1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTEN, BZS, MHAM, TEP1, MMAC1, PTEN1, MGC11227, phosphatase and tensin homolog (mutated in multiple advanced cancers 1), tensin homolog, mutated in multiple advanced cancers 1, MMAC1 phosphatase and tension homolog deleted on chromosome 10
Official Symbol: PTEN
Accession Number(s): NP_000305.3
Human GeneID(s): 5728

Immunogen

Peptide with sequence CNEPFDEDQHTQIT, from the internal region (near the C Terminus) of the protein sequence according to NP_000305.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in lysates of cell line A431 and 50kDa in lysates of cell line NIH3T3 (calculated MW of 47.1kDa according to Human NP_000305 and Mouse NP_032986.1). Recommended concentration: 1-3µg/ml.

Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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