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Goat Anti-RARA Antibody (EB06672)

Related Products: EBP06672 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB06672 Goat Anti-RARA Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB06672 1
EB06672 (4µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06672 2
EB06672 (0.05µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR short form, nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form, Retinoic acid receptor, alpha polypeptide, retinoic acid receptor, alpha, NR1B1, RAR, RARA
Official Symbol: RARA
Accession Number(s): NP_000955.1; NP_001138773.1; NP_001019980.1; NP_001138774.1
Human GeneID(s): 5914
Important Comments: NP_000955.1 (isfa) and NP_001138773.1(alpha) represent identical proteins. This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar.

Immunogen

Peptide with sequence C-SPSLSPSSHRSSPATQSP, from the C Terminus of the protein sequence according to NP_000955.1; NP_001138773.1; NP_001019980.1; NP_001138774.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 50kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 50.8kDa according to NP_000955.1; NP_001019980.1). Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Cerebellum shows staining of Purkinje cytoplasm. Recommended concentration, 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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