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Goat Anti-RFPL2 and RFPL3 Antibody (EB05831)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05831 Goat Anti-RFPL2 and RFPL3 Antibody Pep-ELISA Yes 100µg specific antibody in 200µl Human $210.00 Aspiring

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Target Protein

Principal Names: ret finger protein-like 3, RFPL3, ret finger protein-like 2, RNF79, RFPL2
Official Symbol: RFPL2
Accession Number(s): NP_006596.2; NP_001091997.2; NP_001153017.1; NP_001153018.1; NP_001092005.1; NP_006595.1
Human GeneID(s): 10739 , 10738
Important Comments: This is expected to recognise both RFPL2 (NP_006596.2; NP_001091997.2; NP_001153017.1; NP_001153018.1) and RFPL3 (NP_001092005.1; NP_006595.1), which are virtually identical. Variants (NP_001153017.1; NP_001153018.1) encode the same isoform.

Immunogen

Peptide with sequence C-TTDAPVRPGEAK, from the C Terminus of the protein sequence according to NP_006596.2; NP_001091997.2; NP_001153017.1; NP_001153018.1; NP_001092005.1; NP_006595.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: No signal obtained yet but low background observed in 293, Human Testis and Human Kidney lysates at up to 1µg/ml.

Species Reactivity

Tested:
Expected from sequence similarity: Human

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