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Goat Anti-ribosomal protein L8, Biotinylated Antibody (EB07869-B)

Related Products: EBP07869-B - Immunizing Peptide, EBP07869 - Immunizing Peptide, Goat Anti-ribosomal protein L8 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07869-B Goat Anti-ribosomal protein L8, Biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07869-B 1
Biotinylated EB07869 (0.3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: RPL8, ribosomal protein L8, L8, 60S ribosomal protein L8
Official Symbol: RPL8
Accession Number(s): NP_000964.1
Human GeneID(s): 6132
Non-Human GeneID(s): 26961 (mouse), 26962 (rat)
Important Comments: Reported variants represent identical protein: NP_000964.1, NP_150644.1, NP_001304700.1, NP_001304711.1

Immunogen

Peptide with sequence KAGRAYHKYKAKRNC., from the internal region of the protein sequence according to NP_000964.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28kDa band observed in lysates of cell line HepG2 (calculated MW of 28.0kDa according to NP_000964.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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