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Goat Anti-RIP140 Antibody (EB09546)

Code Name Applications Availability Size Species reactivity Price Grade  
EB09546 Goat Anti-RIP140 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB09546 1
EB09546 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: receptor interacting protein 140, nuclear factor RIP140, OTTHUMP00000083037, OTTHUMP00000083036, RIP140, FLJ77253, nuclear receptor interacting protein 1, NRIP1
Official Symbol: NRIP1
Accession Number(s): NP_003480.2
Human GeneID(s): 8204
Non-Human GeneID(s): 268903 (mouse), 304157 (rat)

Immunogen

Peptide with sequence C-EKDSPRLTKTNP, from the internal region of the protein sequence according to NP_003480.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 140kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 127kDa according to NP_003480.2). Recommended concentration: 0.5-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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