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Goat Anti-RNF23 / TRIM39 Antibody (EB05871)
| Code | Name | Applications | Availability | Size | Species reactivity | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05871 | Goat Anti-RNF23 / TRIM39 Antibody | Pep-ELISA |  |
100µg specific antibody in 200µl | Human, Mouse, Rat, Dog, Pig | $210.00 |  |
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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day. Orders are placed with and invoiced by our US partner Abcore LLC.
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Target Protein
Principal Names: OTTHUMP00000029076, OTTHUMP00000029075, DAAP-385L22.4, testis-abundant finger protein, ring finger protein 23, tripartite motif-containing 39, MGC32984, RNF23, TFP, TRIM39B, TRIM39Official Symbol: TRIM39
Accession Number(s): NP_067076.2; NP_742013.1
Human GeneID(s): 56658
Non-Human GeneID(s): 79263 (mouse)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_067076.2 and NP_742013.1).
Immunogen
Peptide with sequence C-NAAPLTIRPPTDWE, from the C Terminus of the protein sequence according to NP_067076.2; NP_742013.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Preliminary experiments gave an approx 49kDa band in Human Testis and Rat Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_NP_742013.1 and of 59.7kDa according to NP_067076.2. The 49kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species Reactivity
Tested:Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig