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Goat Anti-RNF8 Antibody (EB05843)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05843 Goat Anti-RNF8 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB05843 1
EB05843 staining (0.1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBC13/UEV-interacting ring finger protein, OTTHUMP00000039684, C3HC4-type zinc finger protein, FLJ12013, KIAA0646, ring finger protein (C3HC4 type) 8, ring finger protein 8, RNF8
Official Symbol: RNF8
Accession Number(s): NP_003949.1; NP_898901.1
Human GeneID(s): 9025
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).

Immunogen

Peptide with sequence GEPGFFVTGDRAG-C, from the N Terminus of the protein sequence according to NP_003949.1; NP_898901.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55kDa band observed in Human Placenta and Lung lysates (predicted MW of 56kDa according to NP_003949). Recommended for use at 0.1-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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