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Goat Anti-RXR gamma Antibody (EB06526)

Code Name Applications Availability Size Species reactivity Price Grade  
EB06526 Goat Anti-RXR gamma Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Pig, Cow $315.00 Elite

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Orders are placed with and invoiced by our US partner Abcore LLC.

 

EB06526 1
EB06526 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: retinoid X receptor, gamma, NR2B3, RXRC, RXRG
Official Symbol: RXRG
Accession Number(s): NP_008848.1
Human GeneID(s): 6258
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 213-226 of human RXR gamma protein (hinge region) and does not cross-react with either RXR alpha or beta.

Immunogen

Peptide with sequence RQRSRERAESEAEC, from the internal region of the protein sequence according to NP_008848.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 65kDa band observed in lysates of cell line HeLa (calculated MW of 50.9kDa according to NP_008848.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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