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Goat Anti-Sak / STK18 / PLK4 Antibody (EB05336)

Code Name Applications Availability Size Tested species Price Grade  
EB05336 Goat Anti-Sak / STK18 / PLK4 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $315.00 Elite

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ARP American Research Products, Inc
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EB05336 1
EB05336 (2µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05336 2
EB05336 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05336 3
EB05336 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05336 4
EB05336 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PLK4, Sak, polo-like kinase 4 (Drosophila), serine/threonine kinase 18, SAK, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase, STK18, serine/threonine protein kinase SAK, MCCRP2, STK 18
Official Symbol: PLK4
Accession Number(s): NP_055079.3, NP_001177728.1
Human GeneID(s): 10733

Immunogen

Peptide with sequence ATCIGEKIEDFKVGN, from the N Terminus of the protein sequence according to NP_055079.3, NP_001177728.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110kDa band observed in Human Colon lysates (calculated MW of 109kDa according to NP_055079.3). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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