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Goat Anti-SAP130 / SF3B3 Antibody (EB05543)

Related Products: EBP05543 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05543 Goat Anti-SAP130 / SF3B3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB05543 1
EB05543 staining (0.1µg/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SF3b130, splicing factor 3b, subunit 3, spliceosome-associated protein 130, pre-mRNA splicing factor SF3b, 130 kDa subunit, STAF130, KIAA0017, SF3B130, RSE1, splicing factor 3b, subunit 3, 130kDa, SAP130, SF3B3
Official Symbol: SF3B3
Accession Number(s): NP_036558.3
Human GeneID(s): 23450
Non-Human GeneID(s): 101943 (mouse)

Immunogen

Peptide with sequence C-VSKKLEDIRTRYAF, from the C Terminus of the protein sequence according to NP_036558.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Yoshimoto R, Kataoka N, Okawa K, Ohno M.
Isolation and characterization of post-splicing lariat-intron complexes.
Nucleic Acids Res. 2009 Feb;37(3):891-902.
PMID: 19103666

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