Change country
Home

Goat Anti-SERCA2 / ATP2A2 Antibody (EB11450)

Related Products: EBP11450 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11450 Goat Anti-SERCA2 / ATP2A2 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $450.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

Order offline

Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11450 1
EB11450 (0.1µg/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11450 2
EB11450 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11450 3
EB11450 mmunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: ATP2B, ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2, calcium pump 2, calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, cardiac Ca2+ ATPase, DAR, DD, DKFZp686P0211, endoplasmic reticulum class 1/2 Ca(2+) ATPase, FLJ20293, FLJ38063, MGC45367, sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA2, SR Ca(2+)-ATPase 2, ATP2A2
Official Symbol: ATP2A2
Accession Number(s): NP_001672.1; NP_733765.1
Human GeneID(s): 488
Non-Human GeneID(s): 11938 (mouse), 29693 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).

Immunogen

Peptide with sequence DELNPSAQRDACLN, from the internal region of the protein sequence according to NP_001672.1; NP_733765.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 110kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110kDa according to NP_001672.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Product Reviews

Please login to review this product.