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Goat Anti-SETMAR Antibody (EB05734)

Related Products: EBP05734 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05734 Goat Anti-SETMAR Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Cow $315.00 Elite

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EB05734 1
EB05734 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SET domain and mariner transposase fusion, METNASE, SET domain and mariner transposase fusion gene, SETMAR
Official Symbol: SETMAR
Accession Number(s): NP_006506.3
Human GeneID(s): 6419

Immunogen

Peptide with sequence RWQKCVDCNGSYFD, from the C Terminus of the protein sequence according to NP_006506.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70-75kDa band seen in Daudi lysate. Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

Specific Reference

The goat polyclonal antibody used in this paper was manufactured by us:
Van Duyne R, Easley R, Wu W, Berro R, Pedati C, Klase Z, Kehn-Hall K, Flynn EK, Symer DE, Kashanchi F.
Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTR.
Retrovirology. 2008 May 22;5:40.
PMID: 18498648

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