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Goat Anti-SF3B4 / SAP49 Antibody (EB05540)

Code Name Applications Availability Size Species reactivity Price Grade  
EB05540 Goat Anti-SF3B4 / SAP49 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Pig, Cow $315.00 Elite

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EB05540 1
EB05540 staining (0.1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: splicing factor 3b, subunit 4, pre-mRNA splicing factor SF3b 49 kDa subunit, SF3b49, splicing factor 3b, subunit 4, spliceosomal protein, spliceosome-associated protein (U2 snRNP), MGC10828, SF3B49, splicing factor 3b, subunit 4, 49kD, SAP49, SF3B4
Official Symbol: SF3B4
Accession Number(s): NP_005841.1
Human GeneID(s): 10262
Non-Human GeneID(s): 107701 (mouse)

Immunogen

Peptide with sequence AAGPISERNQDAT-C, from the N Terminus of the protein sequence according to NP_005841.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in Human HeLa lysates (calculated MW of 44.4kDa according to NP005841.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific References

The goat polyclonal antibody used in this paper was manufactured by us:
Yamasaki S, Ishikawa E, Sakuma M, Hara H, Ogata K, Saito T.
Mincle is an ITAM-coupled activating receptor that senses damaged cells.
Nat Immunol. 2008 Oct;9(10):1179-88.
PMID: 18776906

The goat polyclonal antibody used in this paper was manufactured by us:
Friend K, Lovejoy AF, Steitz JA.
U2 snRNP binds intronless histone pre-mRNAs to facilitate U7-snRNP-dependent 3' end formation.
Mol Cell. 2007 Oct 26;28(2):240-52.
PMID: 17964263

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