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Goat Anti-SMAD2 / MADH2 Antibody (EB05147)

Code Name Applications Availability Size Tested species Price Grade  
EB05147 Goat Anti-SMAD2 / MADH2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05147 1
EB05147 (2µg/ml) staining of Mouse Testes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05147 2
EB05147(2µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MADH2, SMAD2, MAD, mothers against decapentaplegic homolog 2 (Drosophila), JV18, MADR2, JV18-1, Mothers against decapentaplegic, Drosophila, homolog of, 2, MAD (mothers against decapentaplegic, Drosophila) homolog 2, SMAD family member 2, MGC22139, MGC34440, hMAD-2, hSMAD2, MAD, mothers against decapentaplegic homolog 2, Mad, mothers against decapentaplegic homolog 2, Mad protein homolog, Mad-related protein 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2
Official Symbol: SMAD2
Accession Number(s): NP_005892.1
Human GeneID(s): 4087

Immunogen

Peptide with sequence SEIWGLSTPNTIDC, from the internal region of the protein sequence according to NP_005892.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 45kDa and 50kDa bands observed in lysates of cell line HeLa, and approx. 50kDa band in Mouse Testis lysates (calculated MWt of 52.3kDa according to Human NP_005892.1 and Mouse NP_001239410.1) Recommended concentration: 1-2µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Cow

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