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Goat Anti-Smooth muscle alpha-actin Antibody (EB06450)

Code Name Applications Availability Size Tested species Price Grade  
EB06450 Goat Anti-Smooth muscle alpha-actin Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat $315.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06450 1
EB06450 (0.01µg/ml) staining of Human (A) and (0.3ug/ml) Rat (B) Duodenum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06450 2
EB06450 (0.1µg/ml) staining of HeLa cell lysate (RIPA buffer, (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06450 3
EB006450 (3.5µg/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.
EB06450 4
EB06450 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. This data is from a previous batch, not on sale.
EB06450 5
EB06450 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: actin, alpha 2, smooth muscle, aorta, OTTHUMP00000020042, ACTA2, ACTSA, AAT6, ACTSA, alpha 2 actin, alpha-cardiac actin, growth-inhibiting gene 4, smooth muscle alpha-actin
Official Symbol: ACTA2
Accession Number(s): NP_001604.1; NP_001135417.1
Human GeneID(s): 59
Important Comments: Variants NP_001604.1 and NP_001135417.1 encode the same protein.

Immunogen

Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1; NP_001135417.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 45kDa band observed in Human and Rat Duodenum lysates and approx. 40-45kDa in lysates oif cell line HeLa (calculated MW of 42kDa according to Human NP_001604.and Rat NP_112266.1). Recommended concentration: 0.01-0.3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular batch.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in IHC on Mouse:
João Paulo M. C. M. Cunha, Gunter Leuckx, Peter Sterkendries, Hannelie Korf, Gabriela Bomfim-Ferreira, Lutgart Overbergh, Bart Vaes, Harry Heimberg, Conny Gysemans, Chantal Mathieu
Human multipotent adult progenitor cells enhance islet function and revascularisation when co-transplanted as a composite pellet in a mouse model of diabetes.
Diabetologia. 2016 Oct 4.
PMID: 27704164

This antibody has been successfully used in WB and IF on Mouse:
Chaofei Li, Tianyi Yu, Yan Liu, Xuelian Chen, and Xiong Zhang.
Topical Application of Insulin Accelerates Vessel Maturation of Wounds by Regulating Angiopoietin-1 in Diabetic Mice.
Int J Low Extrem Wounds. 2015 Dec;14(4):353-64. 
PMID: 26349856

This antibody has been successfully used in IHC on Human:
Zabini D, Crnkovic S, Xu H, Tscherner M, Ghanim B, Klepetko W, Olschewski A, Kwapiszewska G, Marsh LM.
High-mobility group box-1 induces vascular remodelling processes via c-Jun activation.
J Cell Mol Med. 2015 May;19(5):1151-61
PMID: 25726846

This antibody has been successfully used in IHC on Human:
Diana Zabini, Akos Heinemann, Vasile Foris, Chandran Nagaraj, Patrick Nierlich, Zolta´n Ba´lint, Grazyna Kwapiszewska, Irene M. Lang, Walter Klepetko, Horst Olschewski and Andrea Olschewski.
Comprehensive analysis of inflammatory markers in chronic thromboembolic pulmonary hypertension patients.
Eur Respir J. 2014 Oct;44(4):951-62. 
PMID: 25034560

This antibody has been successfully used in IHC on Mouse:
Trokovic N, Pöllänen R, Porola P, Stegaev V, Hetzel U, Tivesten Å, Engdahl C, Carlsten H, Forsblad-d'Elia H, Fagman JB, Lagerquist M, Konttinen YT.
Exosomal secretion of death bullets: a new way of apoptotic escape?
Am J Physiol Endocrinol Metab. 2012 Oct 15;303(8):E1015-24.
PMID: 22912365

This antibody has been successfully used in IF on Human:
McIntire RH, Sifers T, Platt JS, Ganacias KG, Langat DK, Hunt JS.
Novel HLA-G-binding leukocyte immunoglobulin-like receptor (LILR) expression patterns in human placentas and umbilical cords.
Placenta. 2008 Jul;29(7):631-8.
PMID: 18538388

This antibody has been successfully used in IHC on Mouse:
Park SH, Chen WC, Hoffman C, Marsh LM, West J, Grunig G.
Modification of hemodynamic and immune responses to exposure with a weak antigen by the expression of a hypomorphic BMPR2 gene.
PLoS One. 2013;8(1):e55180.
PMID: 23383100

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