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Goat Anti-SNX26 / TCGAP (N Terminus) Antibody (EB07382)

Related Products: EBP05916 - Immunizing Peptide, EBP07382 - Immunizing Peptide, Goat Anti-SNX26 / TCGAP (C Terminus) Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB07382 Goat Anti-SNX26 / TCGAP (N Terminus) Antibody ELISA, IHC Yes 100µg specific antibody in 200µl Human $300.00 Elite

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Toll Free Tel: 1-800-550-3033
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EB07382 1
EB07382 1.25µg/ml) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: FLJ39019, sorting nexin 26, TCGAP, SNX26
Official Symbol: SNX26
Accession Number(s): NP_443180.2
Human GeneID(s): 115703

Immunogen

Peptide with sequence ARSTDSLDGPGE-C, from the N Terminus of the protein sequence according to NP_443180.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35kDa band in human brain (hippocampus) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 121kDa according to NP_443180.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Prostate shows cytoplasm staining of secretory cells in the glands. Recommended concentration, 1.25µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Background Reference

Liu H, Nakazawa T, Tezuka T, Yamamoto T.
Physical and functional interaction of FYN tyrosine kinase with a brain-enriched RHO GTPASE-activating protein TCGAP.
J Biol Chem. 2006 Jun 15; [Epub ahead of print]
PMID: 16777849

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