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Goat Anti-SOCS3 Antibody (EB05019)

Related Products: EBP05019 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05019 Goat Anti-SOCS3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05019 1
EB05019 (1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: SOCS3, CIS3, Cish3, SSI-3, SOCS-3, suppressor of cytokine signaling 3, STAT induced STAT inhibitor 3, cytokine-induced SH2 protein 3, ATOD4, MGC71791, SSI3
Official Symbol: SOCS3
Accession Number(s): NP_003946.3
Human GeneID(s): 9021
Non-Human GeneID(s): 12702 (mouse), 89829 (rat)

Immunogen

Peptide with sequence C-PIREFLDQYDAPL, from the C Terminus of the protein sequence according to NP_003946.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 26kDa band observed in lysates of cell line MOLT4 (calculated MW of 24.8kDa according to NP_003946.3). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse, Rat and Pig Liver for this particular batch.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific Reference

This antibody (previous batch) has been successfully used in Western blot on Rat:
Buckley MM, O'Halloran KD, Rae MG, Dinan TG, O'Malley D.
Modulation of enteric neurons by interleukin-6 and corticotropin-releasing factor contributes to visceral hypersensitivity and altered colonic motility in a rat model of irritable bowel syndrome.
J Physiol. 2014 Dec 1;592(23):5235-50.
PMID: 25260633

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