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Goat Anti-Sorbitol Dehydrogenase Antibody (EB06601)

Code Name Applications Availability Size Tested species Price Grade  
EB06601 Goat Anti-Sorbitol Dehydrogenase Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $315.00 Elite

Ordering Information for

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$49.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06601 1
EB06601 (0.1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06601 2
EB06601 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: sorbitol dehydrogenase, SORD, SORD1
Official Symbol: SORD
Accession Number(s): NP_003095.2
Human GeneID(s): 6652

Immunogen

Peptide with sequence KIMLKCDPSDQNP, from the C Terminus of the protein sequence according to NP_003095.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 38kDa band observed in Mouse Kidney lysates (calculated MW of 38.3kDa according to NP_003095.1). Recommended concentration: 0.1-0.3µg/ml. A minor band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

This antibody has been successfully used in Western blot on Mouse:
Niimi N, Yako H, Takaku S, Kato H, Matsumoto T, Nishito Y, Watabe K, Ogasawara S, Mizukami H, Yagihashi S, Chung SK, Sango K.
A spontaneously immortalized Schwann cell line from aldose reductase-deficient mice as a useful tool for studying polyol pathway and aldehyde metabolism.
J Neurochem. 2018 Mar;144(6):710-722.
PMID: 29238976

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