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Goat Anti-SORL1 / LR11 (C Terminus) Antibody (EB05045)

Related Products: EBP05045 - Immunizing Peptide, EBP07713 - Immunizing Peptide, Goat Anti-SORL1 / LR11 (internal) Antibody
Code Name Applications Availability Size Species reactivity Price Grade  
EB05045 Goat Anti-SORL1 / LR11 (C Terminus) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Dog, Cow $315.00 Elite

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EB05045 1
EB05045 (0.3µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: sortilin-related receptor containing LDLR class A repeats, FLJ39258, FLJ21930, C11orf32, mosaic protein LR11, SorLA-1, gp250, SORLA, LRP9, sortilin-related receptor, L(DLR class) A repeats-containing, LR11, SORL1
Official Symbol: SORL1
Accession Number(s): NP_003096.1
Human GeneID(s): 6653

Immunogen

Peptide with sequence C-TGFSDDVPMVIA, from the C Terminus of the protein sequence according to NP_003096.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 250kDa band observed in Mouse and Rat Brain lysates (calculated MW of 248kDa according to Human NP_003096.1, and 247kDa accordnig to Mouse NP_035566.2). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in the following papers:
Kim E, Lee Y, Lee HJ, Kim JS, Song BS, Huh JW, Lee SR, Kim SU, Kim SH, Hong Y, Shim I, Chang KT.
Implication of mouse Vps26b-Vps29-Vps35 retromer complex in sortilin trafficking.
Biochem Biophys Res Commun. 2010 Dec 10;403(2):167-71.
PMID: 21040701

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