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Goat Anti-SSA1 / Ro52 Antibody (EB05901)

Related Products: EBP05901 - Immunizing Peptide
Code Name Applications Availability Size Species reactivity Price Grade  
EB05901 Goat Anti-SSA1 / Ro52 Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $315.00 Elite

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EB05901 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with EB05901 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB05901 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: tripartite motif protein 21, tripartite motif protein TRIM21, Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sicca syndrome antigen A, 52kDa ribonucleoprotein autoantigen SS-A/Ro, 52kD Ro/SSA autoantigen, RNF81, RO52, SSA, SSA1, tripartite motif-containing 21, TRIM21
Official Symbol: TRIM21
Accession Number(s): NP_003132.2
Human GeneID(s): 6737

Immunogen

Peptide with sequence CPLNIGSQGSTDY, from the C Terminus of the protein sequence according to NP_003132.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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